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Strain of yeast used to produce large amounts of proteins, sugars, or other compounds
A yeast expression platform is a strain of yeast used to produce large amounts of proteins, sugars or other compounds for research or industrial uses.
Yeast_expression_platform
Species of fungus
Enrico Berardi; Marten Veenhuis; Ida van der Klei (2005). "New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris
Ogataea_polymorpha
Indian entrepreneur (born 1953)
when it went public. Biocon continues to expand into new areas. Yeast expression platforms offer a desirable alternative to mammalian cell cultures for the
Kiran_Mazumdar-Shaw
Expression of a gene in an organism previously without it
organism can be a bacterium, yeast, mammalian cell, or plant cell. This host is called the "expression system". Homologous expression, on the other hand, refers
Heterologous_expression
Genus of fungus used industrially and as model organism
Komagataella is a methylotrophic yeast within the order Pichiales. It was found in the 1960s as Pichia pastoris, with its feature of using methanol as
Komagataella
Species of fungus
Candida albicans is an opportunistic pathogenic yeast that is a common member of the human gut flora. It can also survive outside the human body. It is
Candida_albicans
Biotechnological process
Commonly used protein production systems include those derived from bacteria, yeast, baculovirus/insect, mammalian cells, and more recently filamentous fungi
Protein_production
Molecular biology technique
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions
Two-hybrid_screening
Biological cell regulation mechanisms
activator that controls the expression of GAL1, GAL7, and GAL10 (all of which code for the metabolic of galactose in yeast). The GAL4/UAS system has been
Regulation_of_gene_expression
Austro-American scientist
Wittrup at MIT and Errik Anderson to develop a novel platform for the discovery of human antibodies in yeast. Gerngross stepped down from heading Adimab in
Tillman_Gerngross
Compact and highly condensed form of chromatin
the Control of Nuclear RNAi: Lessons from Fission Yeast". RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity. Caister Academic Press
Heterochromatin
Conversion of a gene's sequence into a mature gene product or products
Gene expression is the process by which the information contained within a gene is used to produce a functional gene product, such as a protein or a functional
Gene_expression
Species of fungus
plasmids is similar to that described under Hansenula polymorpha and yeast expression platforms. Here are two special examples of recombinant strains and their
Arxula_adeninivorans
American biologist
an American synthetic biologist whose primary research is in the use of yeast to produce opioids for medical use. After graduating from California Institute
Christina_Smolke
American biochemist
in molecular biology, and his thesis was a genome-wide expression analysis of the budding yeast S. cerevisiae. Upon graduation, DeRisi accepted a position
Joseph_DeRisi
Technique in molecular biology
Michal; Mosquna, Assaf (2022-08-19). "A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein–Protein
Reporter_gene
Pharmaceutical compound
about the toxicity of CRM197, with a 2008 study finding that it is toxic to yeast cells and some mammalian cell lines. CRM197 is a genetically detoxified
CRM197
Major cell cycle checkpoint in yeast
The Start point is a major cell cycle checkpoint in yeast, known as the restriction point in multicellular organisms. The Start checkpoint ensures cell-cycle
Start_point_(yeast)
Protein family
N-ethylmaleimide-Sensitive Factor Attachment Proteins (SNAP, or Sec17p in yeast) are a family of cytosolic adaptor proteins involved in vesicular fusion
Soluble NSF attachment protein
Soluble_NSF_attachment_protein
the Ensembl software platform) FlyBase: genome of the model organism Drosophila melanogaster Gene Disease Database Gene Expression Omnibus (GEO): a public
List_of_biological_databases
Bioinformatics software platform
source bioinformatics software platform for visualizing molecular interaction networks and integrating with gene expression profiles and other state data
Cytoscape
Attachment of an oligosaccharide to a nitrogen atom
prokaryotic expression systems such as E. coli cannot carry out post-translational modifications. On the other hand, eukaryotic expression hosts such as yeast and
N-linked_glycosylation
Multinational biotechnology research organization
microarray products and services used for gene expression profiling and miRNA profiling. Human, Mouse, Rat and Yeast whole genome OneArray DNA microarrays are
Phalanx_Biotech_Group
protocol for the yeast S. cerevisiae (Genomic run-on, GRO) that allows for the calculation of transcription rates (TRs) for all yeast genes to estimate
Nuclear_run-on
Form of protein microarray
using three antibody array platforms: sandwich-based, quantitative, and label-based, and a strong correlation in protein expression was found, suggesting that
Antibody_microarray
Laboratory technique to multiply an RNA sample for study
was hypothesized to selectively abolish Gal expression. To confirm this, gene expression levels of yeast cells containing this mutation were analyzed
Reverse transcription polymerase chain reaction
Reverse_transcription_polymerase_chain_reaction
biological phenomena. His work has spanned from basic cell biology, using yeast as a model system, focusing on understanding molecular mechanisms of nuclear
John_D._Aitchison
Addition of adenylic acids to 3' end of mature mRNA
of the mRNA. It, therefore, forms part of the larger process of gene expression. The process of polyadenylation begins as the transcription of a gene
Polyadenylation
Site-specific recombinase technology
cells". Sauer B (June 1987). "Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae". Molecular and
Cre-Lox_recombination
Vaccine that contains antigenic parts of the pathogen
the expression system of the dengue vaccine. Yeast matches bacterial cells' cost-effectiveness, efficiency and technical feasibility. Moreover, yeast secretes
Subunit_vaccine
Chromatin remodeling complex
Herskowitz, Ira (October 1984). "Five SWI genes are required for expression of the HO gene in yeast". Journal of Molecular Biology. 178 (4): 853–868. doi:10
Mammalian SWI/SNF (BAF) complex
Mammalian_SWI/SNF_(BAF)_complex
Lab technique in cellular biology
gene fusion, mutations/SNPs and changes in gene expression over time, or differences in gene expression in different groups or treatments. In addition
RNA-Seq
Protein-coding gene in the species Homo sapiens
mutation at www.stag1gene.org. SA1 is one of three human homologues of the yeast protein Scc3 which is a core subunit of the cohesin complex (the three human
STAG1
Danish biologist
Kildegaard KR, Li M, Borodina I, Nielsen J (2015). "Establishment of a yeast platform strain for production of p-coumaric acid through metabolic engineering
Jens_Nielsen
Collection of molecular regulators
Transcriptional Regulation Data and Analysis Platform Open source web service for GRN analysis BIB: Yeast Biological Interaction Browser Graphical Gaussian
Gene_regulatory_network
Genetic perturbation technique
perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells. It was first developed by Stanley
CRISPR_interference
Protein-coding gene in the species Homo sapiens
AT, Bird AC, Hunt DM, Bhattacharya SS (August 2001). "A human homolog of yeast pre-mRNA splicing gene, PRP31, underlies autosomal dominant retinitis pigmentosa
PRPF31
International bioinformatics company
subsidiary, Proteome Inc in Beverly, MA. The early flagship of Proteome was the Yeast Proteome Database (YPD), which was complemented by a number of similar databases
Biobase_(company)
spectrometric analysis Protein and peptide microarrays Western blotting Yeast two-hybrid based protein–protein interaction This portal that allows adding
Human_Proteinpedia
Examines sequence information from individual cells
yeast cells. The single-cell yeast study sought to characterize the heterogeneous stress tolerance in isogenic yeast cells before and after the yeast
Single-cell_sequencing
Study of RNA transcripts
a single genome gives rise to a variety of cells. Another is how gene expression is regulated. The first attempts to study whole transcriptomes began in
Transcriptomics_technologies
DNA reverse transcribed from RNA
be transferred to a recipient cell for expression as part of recombinant DNA, often bacterial or yeast expression systems. cDNA is also generated to analyze
Complementary_DNA
Production of protein in a cell-free system
(ECE), rabbit reticulocytes (RRL), wheat germ (WGE), insect cells (ICE) and Yeast Kluyveromyces (the D2P system). All of these extracts are commercially available
Cell-free_protein_synthesis
Subviral agents that lack nucleic acid
Barbian A, Chan JA, et al. (February 2018). "Establishment of a yeast-based VLP platform for antigen presentation". Microbial Cell Factories. 17 (1): 17
Virus-like_particle
Duplication of a gene sequence within a genome
C.; Wolfe, Kenneth H. (April 2006). "Functional partitioning of yeast co-expression networks after genome duplication". PLOS Biology. 4 (4) e109. doi:10
Gene_duplication
this divergence could be responsible for the different targeting of the yeast Ty elements. A mutation was identified in the integrase C-terminus that
Ty5_retrotransposon
Israeli professor of computer science (born 1953)
evolvability in regulatory networks: The evolution of ribosomal regulation in yeast", Proceedings of the National Academy of Sciences USA, 102 (20): 7203–7208
Ron_Shamir
Cellular mechanism
can alter or eliminate the cell's ability for transcription and gene expression. Other lesions may induce potentially harmful mutations in the cell's
DNA_repair
Combination in the membranes of cells
micrometers) that segregate vacuolar proteins into distinct regions in live yeast cells. These domains are influenced by environmental cues (e.g. nutrient
Lipid_raft
Group of methods in synthetic biology
the nucleotide sequence or size. Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis
Artificial_gene_synthesis
Biological manufacturing process
non-conventional yeast with advantages in natural product biosynthesis. It is easier to genetically modify and is well-suited for protein expression. S. cerevisiae
Precision_fermentation
Biological process of gene regulation
which RNA molecules are involved in sequence-specific suppression of gene expression by double-stranded RNA, through translational or transcriptional repression
RNA_interference
American biologist
Fordham University in 1976. She conducted studies of the genetic code in yeast mitochondrial DNA. She earned her PhD in molecular and cell biology at NYU
Gloria_M._Coruzzi
Field of study
The SWI/SNF protein complex in yeast is one example of a chromatin remodeling complex that regulates the expression of many genes through chromatin remodeling
Epigenomics
Transcriptomes of S. purpuratus S. cerevisiae YeastMine transcriptome database. Wheat WheatExp – an RNA-seq expression database for polyploid wheat. Wang Z, Gerstein
List of RNA-Seq bioinformatics tools
List_of_RNA-Seq_bioinformatics_tools
Method used to analyse protein interactions with DNA
melanogaster), yeast, and monkeys, among others. The first study to use this digestion to study the relevance of chromatin accessibility to gene expression in humans
MNase-seq
Rod-shaped, gram-negative bacterium
expression circuit". Nature Biotechnology. 29 (12): 1114–1116. doi:10.1038/nbt.2018. PMC 4565053. PMID 22057053. Sawyer E. "Computer Controlled Yeast
Escherichia_coli
Method in molecular genetics
efficient as in yeast. In the case of the yeast model system directed deletions have been created in every non-essential gene in the yeast genome. In the
Reverse_genetics
Interdisciplinary branch of biology and engineering
ease of application. Engineered yeast-based platform Synthetic biologists are developing genetically modified live yeast that can deliver therapeutic biologic
Synthetic_biology
Preparation for acquired immunity to disease
infected patients but now produced by recombination of the viral genes into yeast). Other examples include the Gardasil virus-like particle human papillomavirus
Vaccine
at CpG island promoters in human cells and highly transcribed regions in yeast. Under abnormal conditions, namely elevated production of DNA-RNA hybrids
DRIP-seq
Class of enzymes
a principal epigenetic modification of chromatin that determines gene expression, genomic stability, stem cell maturation, cell lineage development, genetic
Histone_methyltransferase
Form of eukaryotic RNA polymerase II
for the successful transcription of nearly all class II gene promoters in yeast. It works in the same manner in mammals. The mediator functions as a coactivator
RNA_polymerase_II_holoenzyme
Protein-coding gene in the species Homo sapiens
eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of the pre-replication
ORC1
American scientist
Malone at the University of Iowa, working on the control of recombination in yeast. Following her graduation, she spent a summer working on enhancers in Drosophila
Anne_E._Carpenter
Protein-coding gene in the species Homo sapiens
conservation over a long period of evolution. The solution structure of TCTP from yeast, Schizosaccharomyces pombe has been determined by NMR spectroscopy which
Translationally controlled tumor protein
Translationally_controlled_tumor_protein
Post-translational removal of peptide sequences from a protein sequence
and carrot vacuolar ATPase (without intein) and the homologous gene in yeast (with intein) that was first described as a putative calcium ion transporter
Protein_splicing
American geneticist
the RNR2 gene and protein in yeast, which belongs to the family of ribonucleotide reductase, and found that its expression increases when DNA is damaged
Stephen_Elledge
Bioinformatics company
for microarray gene expression data analysis guides the user throughout a step-by-step workflow. OmicsHub® Proteomics is a platform for the central management
Integromics
level 1 laboratory, primarily working with non-pathogenic organisms such as yeast and E. coli. Its facility includes laboratory benches for experiments, refrigeration
Boston Open Source Science Laboratory
Boston_Open_Source_Science_Laboratory
Antibodies from clones of the same blood cell
phage display/yeast display technologies. Recombinant antibody engineering involves antibody production by the use of viruses or yeast, rather than mice
Monoclonal_antibody
American scientist
the nascent technologies of protein engineering and recombinant protein expression, and then at The Scripps Research Institute in the laboratory of Ian Wilson
K._Christopher_Garcia
Microfluidic systems can be used to incubate high volumes of bacteria and yeast colonies. The two-layer microchemostat device is made to allow scientists
Microfluidic_cell_culture
Alteration in the nucleotide sequence of a genome
majority of mutations are caused by translesion synthesis. Likewise, in yeast, Kunz et al. found that more than 60% of the spontaneous single base pair
Mutation
transformed into a host which is capable of assembling the components (typically yeast or mammalian cells) into a functional chromosome. This approach has been
Minichromosome
Species of fungus
L.; Skatrud, P. L.; Yeh, W. K.; Queener, S. W. (1990). "Cloning and expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum"
Penicillium_rubens
Research tool in genomics
elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide scale and studying the resulting phenotypic alterations
Genome-wide CRISPR-Cas9 knockout screens
Genome-wide_CRISPR-Cas9_knockout_screens
Biomolecule
operating within the RNA interference (RNAi) pathway. It interferes with the expression of specific genes with complementary nucleotide sequences by degrading
Small_interfering_RNA
Linked series of chemical reactions occurring within a cell
This process required 56 genetic edits, including expression of 34 heterologous genes from plants in yeast cells. KaPPA-View4 (2010) Metabolism Metabolic
Metabolic_pathway
is Bakerix, a bread/Gaul-themed villain who can get stronger by drinking yeast from his flask and has teamed up with Qilin, Weredad, and Befana to form
List of Miraculous: Tales of Ladybug & Cat Noir characters
List_of_Miraculous:_Tales_of_Ladybug_&_Cat_Noir_characters
Biological process
H3K36 methylation. In yeast and mammals, H3K36 methylation is highly enriched in the body of highly transcribed genes. In yeast at least, H3K36me3 recruits
DNA_methylation
Repeating sequences of 2–13 base pairs of DNA
Most eukaryotes have microsatellites, with the notable exception of some yeast species. Microsatellites are distributed throughout the genome. The human
Microsatellite
Molecular biology method
all ORFs and intergenic regions from the yeast genome. Nowadays, Affymetrix offers whole-genome tiled yeast arrays with a resolution of 5bp (all in all
ChIP-on-chip
Biological processes used in gene regulation
N-terminal chromodomains. The MYST proteins Esa1, Sas2 and Sas3 are found in yeast, MOF is found in Drosophila and mice while Tip60, MOZ, MORF, and HBO1 are
Histone acetylation and deacetylation
Histone_acetylation_and_deacetylation
Protein-coding gene in the species Homo sapiens
budding yeast (S. cerevisiae) and fission yeast (S. pombe). The POLD1 gene promoter is regulated via the cell cycle machinery and mRNA expression of POLD1
POLD1
Organisms used to study biology across species
discoveries of the 20th and 21st centuries. Other examples include baker's yeast (Saccharomyces cerevisiae), the T4 phage virus, the fruit fly Drosophila
Model_organism
Low temperature dehydration process
Primary applications of freeze drying include biological (e.g., bacteria and yeasts), biomedical (e.g., surgical transplants), food processing (e.g., coffee)
Freeze_drying
Protein complex that regulates the separation of sister chromatids during cell division
Condensin, MukBEF and SMC-ScpAB. Cohesin was separately discovered in budding yeast (Saccharomyces cerevisiae) both by Douglas Koshland and Kim Nasmyth in 1997
Cohesin
Protein–protein interactions in human cells
baits and 5600 preys in a yeast two hybrid system to piece together the interactome, and Rual et al. performed a similar yeast-two hybrid study verified
Human_interactome
Discipline in genetics
1986. In 1992, the first eukaryotic chromosome, chromosome III of brewer's yeast Saccharomyces cerevisiae (315 kb) was sequenced. The first free-living organism
Genomics
Group of drugs used against malaria
precursor compound that can be produced using a genetically engineered yeast, which is much more efficient than extraction from the plant. Artemisinin
Artemisinin
InterPro Family
biopharmaceutical, has been expressed in different expression systems including prokaryotic, protozoan, fungal (yeasts), plant, insect and mammalian cells. Human
Interferon_gamma
Biological technique to evolve proteins using bacteriophages
Platform." Advanced Science. 12: e15356. https://doi.org/10.1002/advs.202415356 . Smith GP (June 1985). "Filamentous fusion phage: novel expression vectors
Phage_display
Any cause that reduces reproductive success in a proportion of a population
gene like HIS4 gene under amino acid selective pressure in yeast causes enhancement of expression of adjacent genes which is due to the transcriptional co-regulation
Evolutionary_pressure
(baker's yeast or budding yeast), used in brewing and baking. Schizophyllum commune, model for mushroom formation. Schizosaccharomyces pombe, fission yeast, (cell
List_of_model_organisms
Biotechnological procedure
method for quantification of residual DNA in protein drugs produced in yeast cells". Journal of Pharmaceutical and Biomedical Analysis. 123: 128–131
Digital polymerase chain reaction
Digital_polymerase_chain_reaction
Protein-coding gene in humans
found in other vertebrates, including mammals, birds, and reptiles. The expression of BEND2 in Homo sapiens is regulated and occurs at high levels in the
BEND2
System that supports a biologically active environment
compared to industrial bioreactors used for growing protein expression systems such as yeast and bacteria.[citation needed] Many research groups have developed
Bioreactor
Protein-coding gene in the species Homo sapiens
eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional
ORC6
Free-living species of nematode
non-contaminated bacteria. C. elegans can also use different species of yeast, including Cryptococcus laurentii and C. kuetzingii, as sole sources of
Caenorhabditis_elegans
YEAST EXPRESSION-PLATFORM
YEAST EXPRESSION-PLATFORM
Girl/Female
Indian
Expressions
Surname or Lastname
English
English : topographic name for someone who lived in the eastern part of a town or settlement, or outside it to the east, or a regional name for someone who had migrated from the east of a place. As an American family name, this surname has absorbed various other European names with similar meaning.
Boy/Male
Celebrity, Gujarati, Hindu, Indian, Kannada, Telugu
Art of Expressing Acting; Expression
Boy/Male
Muslim
Expression
Girl/Female
Sikh
Expression
Boy/Male
Indian
Expression
Girl/Female
Indian
Cheerful expression
Girl/Female
Indian
Expressions
Boy/Male
Arabic, Muslim, Pashtun
Expression
Boy/Male
Tamil
Expression
Surname or Lastname
English
English : unexplained.
Girl/Female
Tamil
Expressions
Girl/Female
Tamil
Expression
Girl/Female
Hindu, Indian
Expressions
Biblical
which is before or in front of a person
Girl/Female
Tamil
Cheerful expression
Girl/Female
Muslim/Islamic
Grace Expression
Girl/Female
Tamil
Abbynayha | அபà¯à®ªà¯à®¯à¯à®¨à®¾à®¯à¯à®¹à®¾
Expressions
Abbynayha | அபà¯à®ªà¯à®¯à¯à®¨à®¾à®¯à¯à®¹à®¾
Boy/Male
Indian, Sanskrit
Expression
Surname or Lastname
English
English : unexplained.
YEAST EXPRESSION-PLATFORM
YEAST EXPRESSION-PLATFORM
Boy/Male
Hindu, Indian, Marathi, Traditional
Light of Knowledge
Girl/Female
Danish, Hindu, Indian, Sanskrit, Tamil
Body of Water
Girl/Female
Muslim
Gazelle
Boy/Male
Hindu
Lord Vishnu, Lord Krishna
Boy/Male
Hindu
Quick
Girl/Female
Tamil
City
Boy/Male
Gujarati, Hindu, Indian, Kannada, Malayalam, Marathi, Sindhi, Telugu
Fearless King
Girl/Female
Gujarati, Hindu, Indian, Sikh
Truthful; Blessings
Boy/Male
Arthurian Legend
A knight.
Girl/Female
Latin
Venus's surname.
YEAST EXPRESSION-PLATFORM
YEAST EXPRESSION-PLATFORM
YEAST EXPRESSION-PLATFORM
YEAST EXPRESSION-PLATFORM
YEAST EXPRESSION-PLATFORM
n.
The foam, or troth (top yeast), or the sediment (bottom yeast), of beer or other in fermentation, which contains the yeast plant or its spores, and under certain conditions produces fermentation in saccharine or farinaceous substances; a preparation used for raising dough for bread or cakes, and making it light and puffy; barm; ferment.
n.
The act of expressing; expression; utterance; representation.
a.
Frothy; foamy; spumy, like yeast.
n.
A form of words in which an idea or sentiment is conveyed; a mode of speech; a phrase; as, a common expression; an odd expression.
n.
The act of expressing; the act of forcing out by pressure; as, the expression of juices or oils; also, of extorting or eliciting; as, a forcible expression of truth.
n.
Lively or vivid representation of meaning, sentiment, or feeling, etc.; significant and impressive indication, whether by language, appearance, or gesture; that manner or style which gives life and suggestive force to ideas and sentiments; as, he reads with expression; her performance on the piano has expression.
a.
Toward the rising sun; or toward the point where the sun rises when in the equinoctial; as, the east gate; the east border; the east side; the east wind is a wind that blows from the east.
n.
The act of declaring or signifying; declaration; utterance; as, an expression of the public will.
a.
Full of expression; vividly representing the meaning or feeling meant to be conveyed; significant; emphatic; as, expressive looks or words.
a.
Of, or relating to, expression; phraseological; also, vividly representing or suggesting an idea or sentiment.
pl.
of Expressman
n.
A sense of heaviness or obstruction in the body or mind; depression; dullness; lassitude; as, an oppression of spirits; an oppression of the lungs.
v. i.
To move toward the east; to veer from the north or south toward the east; to orientate.
n.
The eastern parts of the earth; the regions or countries which lie east of Europe; the orient. In this indefinite sense, the word is applied to Asia Minor, Syria, Chaldea, Persia, India, China, etc.; as, the riches of the East; the diamonds and pearls of the East; the kings of the East.
a.
A term used of beer when the froth of the yeast has reentered the body of the beer.
n.
A form of fungus which grows as indvidual rounded cells, rather than in a mycelium, and reproduces by budding; esp. members of the orders Endomycetales and Moniliales. Some fungi may grow both as a yeast or as a mycelium, depending on the conditions of growth.
v. t.
To delight; to gratify; as, to feast the soul.
n.
See Yeast.
n.
A penalty at beast, omber, etc. Hence: To be beasted, to be beaten at beast, omber, etc.
a.
Smallest, either in size or degree; shortest; lowest; most unimportant; as, the least insect; the least mercy; the least space.