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Ability of a substance to resist changes in structure under high temperatures
particular protein is responsible for its thermostability. Another type of force that is responsible for thermostability of a protein is the electrostatic interactions
Thermostability
Microorganisms which live in water with high temperature and high acidity
rings attached to their lipids that have been shown to increase the thermostability of the membrane. Despite all of the previously mentioned adaptations
Thermoacidophile
DNA polymerases that originate from thermophiles
introducing Taq polymerase for PCR. The thermostability of Taq polymerase obliviated the need to add a non-thermostable DNA polymerase to the reaction after
Thermostable_DNA_polymerase
Thermostable form of DNA polymerase I used in polymerase chain reaction
with proof-reading ability but a lower optimal temperature and low thermostability. Versions of the polymerase without the 5'-3' exonuclease domain has
Taq_polymerase
Type of animal that produces metabolic heat, but has no specific body temperature
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Mesotherm
Animal species that can maintain a body temperature higher than their environment
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Warm-blooded
Chemical property of substances
their applications in DNA replication techniques, such as PCR, where thermostable enzymes are necessary for proper DNA replication. Enzyme function at
Thermolabile
Organism where internal heating sources are small or negligible
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Ectotherm
Ability of an organism to keep its body temperature within certain boundaries
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Thermoregulation
System of measuring the instability of a protein under varying conditions
in. For fluorescence-detection size-exclusion chromatography-based thermostability assay (FSEC-TS) the samples are heated in the same manner as in FastPP
Thermal_shift_assay
Metabolic system
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Heterothermy
Organism with considerable internal temperature variation
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Poikilotherm
Form of thermoregulation by body size
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Gigantothermy
Study of the effects of temperature on biology
is required for DNA strand separation without denaturing. Use of the thermostable Taq enables running the PCR at high temperature (~60 °C and above), which
Thermobiology
Organism that thrives at relatively high temperatures
"Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability". Microbiology and Molecular Biology Reviews. 65 (1): 1–43. doi:10
Thermophile
Organisms with a high active metabolism
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Bradymetabolism
Enzyme
100% identical to Pfu DNA polymerase. Pfu DNA polymerase has superior thermostability and proofreading properties compared with Taq DNA polymerase. Unlike
Pfu_DNA_polymerase
Laboratory technique to multiply a DNA sample for study
oligonucleotides that are a complementary sequence to the target DNA region) and a thermostable DNA polymerase. In the first step of PCR, the two strands of the DNA
Polymerase_chain_reaction
Term used to describe animals with low levels of oxygen consumption
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Bradyaerobic
Protein-coding gene in humans
phenol sulfotransferase: effect of mutating cys70 on activity and thermostability". Chemico-Biological Interactions. 92 (1–3): 57–66. doi:10.1016/0009-2797(94)90053-1
SULT1A1
Methods to reduce failure and increase the reliability of PCR
properties of the chimeric polymerases TopoTaq and PfuC2 include enhanced thermostability, specificity and resistance to contaminants and inhibitors. They were
Polymerase chain reaction optimization
Polymerase_chain_reaction_optimization
Species of archaeon
furiosus has many potential industrial applications, owing to its unique thermostable properties. P. furiosus is used in the process of DNA amplification by
Pyrococcus_furiosus
Class of enzymes
thermal cycles (94 °C/80 °C) or 16 hours of cycling.10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity
DNA_ligase
Semicrystalline thermoplastic with high mechanical and chemical resistance
and is commonly used to produce high-quality plastic parts that are thermostable and both electrically and thermally insulating. Filled grades of PEEK
Polyether_ether_ketone
Organism that maintains body temperature largely by heat from internal bodily functions
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Endotherm
and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI)
Polymerase-endonuclease amplification reaction
Polymerase-endonuclease_amplification_reaction
bridges. Mabinlin-2 is the sweet-tasting protein with the highest known thermostability, which is due to the presence of the four disulfide bridges. It has
Mabinlin
combinations of desirable characteristics encoded in the parent genes such as thermostability and high activity. The potential for DNA shuffling to produce novel
DNA_shuffling
Pathogenic type of misfolded protein
et al. (March 2014). "Proteolysis of abnormal prion protein with a thermostable protease from Thermococcus kodakarensis KOD1". Applied Microbiology and
Prion
Naturally produced monosaccharide
Bacillus licheniformis or Bacillus subtilis (strain MN-385), which are more thermostable than the originally used enzymes. Starting in 1982, pullulanases from
Glucose
Maintaining stable internal body temperature
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Homeothermy
Poisonous mushroom (death cap)
poisoning. Amatoxins, the class of toxins found in these mushrooms, are thermostable: they resist changes due to heat and cold, so their toxic effects are
Amanita_phalloides
Species of bacterium
motility or remain immotile. T. aquaticus has become famous as a source of thermostable enzymes, particularly the Taq DNA polymerase, as described below. Studies
Thermus_aquaticus
Chemical compound
Solvent Black 3 is an azo dye. It is a non-fluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides
Solvent_Black_3
physiologically superior genotypes from within bulks. (C) Membrane thermostability Although resistance to high temperatures involves several complex tolerance
Breeding for heat stress tolerance
Breeding_for_heat_stress_tolerance
Type of antibiotic
basic compound that is most active with an alkaline reaction. It is also thermostable and soluble in water (while insoluble in organic solvents). Neomycin
Neomycin
Harmful effects from ingestion of toxic substances present in a mushroom
methods of preparation for cooking. Some toxins, such as amatoxins, are thermostable and mushrooms containing such toxins will not be rendered safe to eat
Mushroom_poisoning
Low temperature dehydration process
original (PDF) on October 21, 2013. Wilding, Kristen (25 November 2019). "Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free
Freeze_drying
Biological process
polymerizes partner strands in each direction from these primers using a thermostable DNA polymerase. Repeating this process through multiple cycles amplifies
DNA_replication
Domain of organisms
these harsh conditions. These enzymes have found many uses. For example, thermostable DNA polymerases, such as the Pfu DNA polymerase from Pyrococcus furiosus
Archaea
Engineering thermoplastic polymer
macromolecules, which he characterized as polymers. Due to problems with thermostability, POM was not commercialized at that time. Circa 1952, research chemists
Polyoxymethylene
Species of bacterium
isolated. It was found during a survey in search of bacteria that produce thermostable collagenase. The optimum growth temperature for A. sendaiensis is 55 °C
Alicyclobacillus_sendaiensis
Species of bacterium
Ghorbel-Frikha, Basma; Kanoun, Safia; Nasri, Moncef (2008-05-01). "Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid
Bacillus_licheniformis
Species of bacterium
Nishikawa, Atsushi; Tonozuka, Takashi (15 March 2014). "Enhancing thermostability and the structural characterization of Microbacterium saccharophilum
Microbacterium_saccharophilum
Type of vaccine
LNP formulations or mRNA secondary structures could account for the thermostability differences [between Moderna and BioNtech], many experts suspect both
MRNA_vaccine
Protein detection method
3'exonuclease proficient polymerases lowers background noise and hyper thermostable polymerases mediate a simple assay with a natural hot-start reaction
Proximity_extension_assay
Use of related sequences to construct an ancestral-like gene
minor protein thermostability throughout the phylogeny – leading to an additive effect resulting in 'superior' ancestral thermostability. Experimental
Ancestral sequence reconstruction
Ancestral_sequence_reconstruction
Extremophile species of bacterium
PMID 26909071. Farci D, Slavov C, Piano D (2018). "Coexisting properties of thermostability and ultraviolet radiation resistance in the main S-layer complex of
Deinococcus_radiodurans
Species of bacteria
environments. Additionally, E. aurantiacum has been found to produce a thermostable protease that functions in alkaline environments. Exiguobacterium aurantiacum
Exiguobacterium_aurantiacum
Chemical compound
resistivity at room temperature is 107 Ω·cm. Ytterbium hydride has a high thermostability. Ytterbium hydride can be produced by reacting ytterbium with hydrogen
Ytterbium(II)_hydride
Fast parallel proteolysis (FASTpp) is a method to determine the thermostability of proteins by measuring which fraction of protein resists rapid proteolytic
Fast_parallel_proteolysis
Swedish scientist (born 1943)
catalytic activity with the anticancer prodrug Telcyta and improved thermostability". Cancers. 16 (4): 762. doi:10.3390/cancers16040762. PMC 10887215.
Bengt_Mannervik
Chemical compound
is produced by calcining zirconium compounds, exploiting its high thermostability. Three phases are known: monoclinic below 1170 °C, tetragonal between
Zirconium_dioxide
Natural explosive eruption of hot water
"Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability". Microbiology and Molecular Biology Reviews. 65 (1): 1–34. doi:10
Geyser
Byproduct in PCR formed by primer annealing to itself
primers: a method known as RNase H-dependent PCR (rhPCR), utilizes a thermostable RNase HII to remove a blocking group from the PCR primers at high temperature
Primer_dimer
Class of polymers
alipharomatic), Aromatic: these are the most used polyimides because of their thermostability. According to the type of interactions between the main chains, polyimides
Polyimide
Species of virus
well characterised to atomic resolution, and the viral particles are thermostable. The identification of the virus is attributed to Lister and Thresh in
Cowpea_mosaic_virus
Species of alga
Thermophilic microalgae have gained biotechnological interest as a source for thermostable enzymes and commercial interest as a source for astaxanthin. C. nivalis
Chlamydomonas_nivalis
Organism tolerant of a wide temperature range
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Eurytherm
other polymerase chain reaction processes, the DNA is amplified by the thermostable DNA polymerase: A target region with an internal section of known sequence
Inverse polymerase chain reaction
Inverse_polymerase_chain_reaction
Anaerobic, hyperthermophilic archaeon
of P. yayanosii interplay in thermostability. These residues allow for molecular maintenance and increased thermostability through supporting a tightly
Pyrococcus_yayanosii
Category of polymers, in which the monomers are joined together by ester links
increases their glass transition temperature, melting temperature, thermostability, chemical stability, and solvent resistance. Polyesters can also be
Polyester
Species of archaeon
(September 2014). "An intermolecular disulfide bond is required for thermostability and thermoactivity of β-glycosidase from Thermococcus kodakarensis
Thermococcus_kodakarensis
Form of thermoregulation in which an animal shares in the heat production of another
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Kleptothermy
Arrangement of amino acid sequence
engineered in the laboratory to improve their catalytic activity or thermostability, or to investigate properties of the original protein. Traditional
Circular permutation in proteins
Circular_permutation_in_proteins
Family of viruses
DNA genomes that infect archaea. The viruses of this family are highly thermostable and can act as a template for site-selective and spatially controlled
Rudiviridae
Polymer with a backbone of one composite and random branches of another composite
grafting methods for copolymer synthesis results in materials that are more thermostable than their homopolymer counterparts. There are three methods of synthesis
Graft_polymer
Organosulfur chemical compound used as a solvent
amplification) or DNA templates with high GC-content (to decrease thermostability). For example, 10% final concentration of DMSO in the PCR mixture with
Dimethyl_sulfoxide
Entropy associated with a molecule's possible conformations
stabilizes the native states. A correlation has been observed between the thermostability of a protein and its proline residue content. Configuration entropy
Conformational_entropy
District in Galmudug, Somalia
Kadeppagari, Ravi-Kumar (2012-04-23). "Evidence for the improvement of thermostability of the maltogenic α-amylase ofAspergillus nigerby negative pressure"
Herale
DNA profiling technique
cloned DNA, and complementary DNA (cDNA) populations. DAF relies on a thermostable DNA polymerase (e.g. Taq polymerase or truncated versions of the enzyme
DNA amplification fingerprinting
DNA_amplification_fingerprinting
tb03149.x. Outtrup H, Norman BE (1984). "Properties and application of a thermostable maltogenic amylase produced by a strain of Bacillus modified by recombinant-DNA
Glucan 1,4-alpha-maltohydrolase
Glucan_1,4-alpha-maltohydrolase
Species of bacterium
GC-content of this bacterium is about 69%, this contributes to the thermostability of this bacterium's genome. The two most widely used strains in laboratory
Thermus_thermophilus
Organism requiring a specific ambient temperature
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Stenotherm
346–353. Tajima M, Urabe I, et al. (1976). "Role of calcium ions in the thermostability of thermolysin and Bacillus subtilis var. amylosacchariticus neutral
Thermolysin
Class of proteins
binding of carbenicillin to the active site increases the enzyme's thermostability with conformational changes. The first carboxylate group in carbenicillin
Penicillin-binding_proteins
Tiwari, Virendra K.; Berkowitz, David B. (2024). "Exploiting Archaeal/Thermostable Enzymes in Synthetic Chemistry: Back to the Future?". ChemCatChem. 16
David_B._Berkowitz
Protein-coding gene in the species Homo sapiens
members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Two alternatively spliced variants that encode the same
SULT1A2
Amino acid
Kumar S, Tsai CJ, Nussinov R (March 2000). "Factors enhancing protein thermostability". Protein Engineering. 13 (3): 179–91. doi:10.1093/protein/13.3.179
Lysine
Various uses and techniques of polymerase chain reaction in the laboratory
with a cleavable block on the 3' end that is removed by the action of a thermostable RNase HII enzyme. There are several DNA polymerases that are used in
Variants_of_PCR
Taiwanese molecular biologist and neuroscientist (1950–2026)
Klenow fragment of Escherichia coli DNA polymerase I, which was not thermostable. Because each cycle required high-temperature denaturation, the polymerase
Alice_Chien_Chang
Proteins that transport oxygen throughout the bodies of some invertebrate animals
T, Hinz HJ, Penz F, Hoff R, Föll R, Decker H (May 1995). "Extreme thermostability of tarantula hemocyanin". FEBS Letters. 364 (1): 9–12. doi:10
Hemocyanin
Species of bacterium
the high temperature (96 °C) step required to denature DNA. In 2013, a thermostable group II intron reverse transcriptase (TGIRT), GsI-IIC-MRF, from G. stearothermophilus
Geobacillus stearothermophilus
Geobacillus_stearothermophilus
Chemical compound
cis-trans isomerism, it has over 1021 alternative stereoisomers. It is thermostable, and boiling contaminated seafood does not noticeably reduce toxicity
Palytoxin
Method of DNA amplification
that differs from polymerase chain reaction (PCR) in that it involves a thermostable ligase to join two probes or other molecules together which can then
Ligase_chain_reaction
Protein created by joining other proteins into a single polypeptide
hydrophilic interactions in the solute to increase protein solubility. Thermostability: Singular peptides or protein fragments are typically added to reduce
Fusion_protein
instability of FLP recombinase, he and Frank Buchholz engineered the thermostable variant FLPe. To support large-scale genome engineering, Stewart and
A._Francis_Stewart
Tripartite protein complex secreted by virulent strains of Bacillus anthracis
octameric pre-channel structure. The octameric form was shown to be more thermostable than the heptameric form, and hence the octameric oligomer can persist
Anthrax_toxin
L; Li, Z; Ni, J; Sheng, D; Shen, Y (September 2007). "A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic
Glycerate_dehydrogenase
InterPro Family
Nucleoplasmin (NPM2), the first identified molecular chaperone, is a thermostable acidic protein with a pentameric structure. The protein was first isolated
Nucleoplasmin
Chemical compound
nickel-cobalt-aluminum (NCA) or nickel-cobalt-manganese (NCM) oxides. Issues with thermostability are better for LiCoO 2 cathodes than other nickel-rich chemistries
Lithium_cobalt_oxide
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Tachyaerobic
Class of enzymes
these temperatures, and one focus of research has been engineering more thermostable versions of xylose isomerase and the other enzymes in the process. The
Xylose_isomerase
Vaccine candidate against COVID-19
approved for clinical trials in China. Manufactured as a liquid, ARCoV is thermostable at room temperature for at least 1 week. Reuters later reported that
Walvax_COVID-19_vaccine
Structural carbohydrate in the cell walls of land plants and some algae
melt and reform, whereas non-amidated low methoxyl pectins can form thermostable gels that withstand high temperatures; these properties make low methoxyl
Pectin
Medication to induce abortion and treat ulcers
rates of side effects compared to other routes. It is inexpensive and thermostable (not requiring refrigeration like oxytocin), making it a cost-effective
Misoprostol
RNA COVID-19 vaccine
LNP formulations or mRNA secondary structures could account for the thermostability differences [between Moderna and BioNtech], many experts suspect both
Moderna_COVID-19_vaccine
Class of enzymes which synthesize nucleic acid chains or polymers
achieve changes from incremental tweaks like higher speed/accuracy/thermostability or major shifts such as conversion of template and product types. All
Polymerase
Biological manufacturing process
Mukherjee, Koel; Nigam, Vinod Kumar (2025-04-03). "Improved production of thermostable pectinase from novel Bacillus siamensis (BIOSMNF45) using computational-conventional
Precision_fermentation
Species of prokaryote
requires reduced methyl viologen. It is theorized that P. fumarii thermostability is due to the accumulation of unusual organic solutes. One of these
Pyrolobus_fumarii
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THERMOSTABILITY
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