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The plant-specific insert (PSI) or plant-specific sequence (PSS) is an independent domain, exclusively found in plants, consisting of approximately 100
Plant-specific_insert
Protein family
albumin from plant seeds This enzyme is present in barley grain and other plants. It is an aspartic protease with a plant-specific insert. Runeberg-Roos
Phytepsin
Protein domain
domain for targeting the correct intracellular compartment. The plant-specific insert is an unusual variation on the SapB domains. It features a circular
Saposin_protein_domain
Arrangement of amino acid sequence
with the plant-specific insert (PSI). The PSI is a domain exclusively found in plants, consisting of approximately 100 residues and found in plant aspartic
Circular permutation in proteins
Circular_permutation_in_proteins
Genetic technique that uses homologous recombination to change an endogenous gene
is used routinely in plant genome engineering to insert specific sequences, with the first published example of GT in plants in the 1980s. However,
Gene_targeting
Methods used to change the DNA of organisms
and plant genomes. Techniques have been devised to insert, delete, and modify DNA at multiple levels, ranging from a specific base pair in a specific gene
Genetic engineering techniques
Genetic_engineering_techniques
Manipulation of an organism's genome
as inserting genes, the process can be used to remove, or "knock out", genes. The new DNA can either be inserted randomly or targeted to a specific part
Genetic_engineering
Science of changing the traits of plants
plant are what determine what type of qualitative or quantitative traits it will have. Plant breeders strive to create a specific outcome of plants and
Plant_breeding
Diseases of plants
gall diseases in many plants, can be provided by dipping cuttings in suspensions of Agrobacterium radiobacter before inserting them in the ground to take
Plant_disease
relevant to botany and plants in general. Terms of plant morphology are included here as well as at the more specific Glossary of plant morphology and Glossary
Glossary_of_botanical_terms
Species of wild onion
Kewensis, a catalog of plants cultivated in London's Kew botanic garden. The species had been introduced to Britain in 1770. The specific epithet tricoccum
Allium_tricoccum
In Molecular biology, an insert is a piece of DNA that is inserted into a larger DNA vector by a recombinant DNA technique, such as ligation or recombination
Insert_(molecular_biology)
Biological particle which transfers genetic material between cells
The vector itself generally carries a DNA sequence that consists of an insert (in this case the transgene) and a larger sequence that serves as the "backbone"
Vector_(molecular_biology)
Small piece of maintainable DNA
vectors using DNA ligase. The target DNA sequence can be inserted into the vector in a specific direction if so desired. The restriction sites may be further
Cloning_vector
Immunity strategy in living beings
of a plant becomes infected with a microbial or viral pathogen, in case of an incompatible interaction triggered by specific elicitors, the plant produces
Innate_immune_system
Unit of the U.S. National Security Agency
gives access to fiber optic links for QUANTUM cooperation. TAO's QUANTUM INSERT technology was passed to UK services, particularly to GCHQ's MyNOC, which
Tailored_Access_Operations
Genus of parasitic plants
itself to a plant, it wraps itself around it. If the host contains food beneficial to dodder, the dodder produces haustoria that insert themselves into
Cuscuta
Superfamily of insects
using the antennae; if the plant smells right, the next action is probing the surface upon landing. The stylus is inserted and saliva secreted, the sap
Aphid
Family of flowering plants
filament can be filiform or flat. The stamens can be inserted inside the coralline tube or exserted. The plants demonstrate simultaneous microsporogenesis, the
Solanaceae
Type of genetic engineering
DNA is inserted, deleted, modified or replaced in the genome of a living organism. Unlike early genetic engineering techniques that randomly insert genetic
Genome_editing
Collection of the total genomic DNA from a single organism
amplification and retrieval of specific clones from the library for analysis. There are several kinds of vectors available with various insert capacities. Generally
Genomic_library
Quaternary ammonium cation
diarrhea treatment, with the package insert claiming efficacy against E. coli and Shigella spp. The Chinese package insert contraindicates berberine for people
Berberine
Concentration of water vapour in the air
measurements of humidity are widely employed: absolute, relative, and specific. Absolute humidity is the mass of water vapor per volume of air (in grams
Humidity
Enzymes that cleave DNA in specific ways
nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made by fusing a TAL effector DNA-binding domain
Transcription activator-like effector nuclease
Transcription_activator-like_effector_nuclease
Process of producing individual organisms with identical genomes
between the insert and vector to cause recombination. Whether restriction/ligation or another method is used, the vector with the insert of interest is
Cloning
Device used in genetic engineering
with the use of Agrobacterium tumefaciens and its Ti plasmid to insert DNA into plant cells. See transformation for different methods of transformation
Gene_gun
Species of mantis camouflaged to resemble flowers to lure their prey
positioning themselves on a plant and either inserting themselves within the irradiance or on the foliage of the plants until a prey insect comes within
Flower_mantis
Soviet nuclear engineer in charge during the Chernobyl disaster (1931–1995)
engineer who was the deputy chief engineer for the Chernobyl Nuclear Power Plant. He supervised the safety test which resulted in the 1986 Chernobyl disaster
Anatoly_Dyatlov
Citrus fruit
incompatible with grapefruit are marked as such on the container or package insert. In 2023, world production of grapefruits (combined with pomelos) was 9
Grapefruit
Investigations into the Chernobyl nuclear accident
occurred in the early hours of 26 April 1986, at the Chernobyl Nuclear Power Plant in Soviet Ukraine. The accident occurred when Reactor Number 4 exploded
Investigations into the Chernobyl disaster
Investigations_into_the_Chernobyl_disaster
Scientific study of plant diseases
diseases in many plants, by dipping cuttings in suspensions of Agrobacterium radiobacter before inserting them in the ground to take root. Plant pathology has
Plant_pathology
Sexual penetration without the use of a condom
OCLC 111715901, PMID 15838192, S2CID 46184888 Nicolas Sheon and Aaron Plant, "Protease Dis-Inhibitors? The Gay Bareback Phenomenon," managingdesire
Bareback_(sexual_act)
Organism altered by genetic engineering
multi-step process. Genetic engineers must isolate the gene they wish to insert into the host organism and combine it with other genetic elements, including
Genetically_modified_organism
bacterium, and Agrobacterium tumefaciens, a plant-virulent bacterium used to insert the recombinant DNA into plants. Plant transformation vectors contain three
Plant_transformation_vector
Species of flowering plant in the family Bromeliaceae
pineapple (Ananas comosus) is a tropical plant with an edible fruit; it is the most economically significant plant in the family Bromeliaceae. The pineapple
Pineapple
Genus of flowering plants
but red to yellow in some species. Stamens, usually ten, rarely eight, insert at the junction of the floral tube and ovary wall, with filaments subulate
Saxifraga
Species of pitcher plant from Borneo
Nepenthes rajah /nɪˈpɛnθiːz ˈrɑːdʒə/ is a carnivorous pitcher plant species of the family Nepenthaceae. It is endemic to Mount Kinabalu and neighbouring
Nepenthes_rajah
Electricity generation by nuclear fusion
breakeven (a net electricity-producing plant) or economic breakeven (where the net electricity pays for the plant's whole-life cost). Thermonuclear fusion
Fusion_power
Biological process
where the ova remain in the female's body and the sperm must somehow be inserted. All known terrestrial arthropods use internal fertilization. Opiliones
Sexual_reproduction
Industrial accident in North Lincolnshire, England (1974)
The Flixborough disaster was an explosion at a chemical plant close to the village of Flixborough, North Lincolnshire, England, on Saturday, 1 June 1974
Flixborough_disaster
Gene or genetic material that has been transferred from one organism to another
organism to fit a specific need is not a new science. However, until the late 1900s farmers and scientists could breed new strains of a plant or organism only
Transgene
Signal generator which outputs video waveforms
signal or plant is compliant with the appropriate analog transmission standards Flat fields, a signal consisting of nothing but a specific color (typically
Video-signal_generator
Class of enzymes that divide DNA
or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction
Restriction_enzyme
Variation of the bacteria Pseudomonas syringae
syringae's DNA with restriction enzymes. Insert the individual DNA pieces into a plasmid. Pieces will insert randomly, allowing for different variations
Ice-minus_bacteria
Subfamily of aphids
"fluffy gnats".[citation needed] Woolly aphids feed by inserting their needle-like mouthparts into plant tissue to withdraw sap. They are able to feed on leaves
Eriosomatinae
Species of pitcher plant from Borneo
Nepenthes villosa /nɪˈpɛnθiːz vɪˈloʊzə/, or the villose pitcher-plant, is a tropical pitcher plant endemic to Mount Kinabalu and neighbouring Mount Tambuyukon
Nepenthes_villosa
Species of virus
foreign genes which have been artificially inserted into the host plant. It is inserted into transgenic plants in a form which is different from that found
Cauliflower_mosaic_virus
Study of genes and heredity in plants
needle, the injection site is highly specific, allowing a large amount of DNA to be inserted directly into a specific location such as the cytoplasm or nucleus
Plant_genetics
Food that aids immunity against a disease
grow the plants and propagate the genetic line. The entire gene is inserted into a plant transformation vector to allow transcription or the epitope within
Edible_vaccine
Constructed heat and power generation facility
Block heat and power plants (BHPPs) are modular facilities or installations that generate both electrical energy and heat for local heat requirements.
Block_heat_and_power_plant
This list of sequenced plant genomes contains plant species known to have publicly available complete genome sequences that have been assembled, annotated
List of sequenced plant genomes
List_of_sequenced_plant_genomes
Genetic engineering technology
make specific changes within plant DNA in order to change its traits, and these modifications can vary in scale from altering single base, to inserting or
New_Breeding_Techniques
Laboratory technique to multiply a DNA sample for study
reaction (PCR) is a laboratory method widely used to amplify copies of specific DNA sequences rapidly, to enable detailed study. PCR was invented in 1983
Polymerase_chain_reaction
1986 nuclear accident in the Soviet Union
On 26 April 1986, reactor 4 of the Chernobyl Nuclear Power Plant, located near Pripyat, Ukraine, exploded. With dozens of direct casualties and thousands
Chernobyl_disaster
Hierarchical level in biological classification
ranks, see Willis & McElwain (2002). These are movable ranks, most often inserted between the class and the legion or cohort. Nevertheless, their positioning
Taxonomic_rank
1983, an antibiotic resistant gene was inserted into tobacco, leading to the first genetically engineered plant. Advances followed that allowed scientists
History of genetic engineering
History_of_genetic_engineering
Sequel to the Berzerk video game
has a specific effect on gameplay for that one room. The order is: Big Otto, Power Plant, Central Computer, and Robot Factory. The Power Plant and the
Frenzy_(1982_video_game)
Type of Soviet nuclear power reactor
the БАЗ or BAZ system, (rapid reactor emergency protection) which would insert 24 uniformly distributed rods into the reactor core via a modified drive
RBMK
Measure of energy in a thermodynamic system
expressed in other specific quantities by h = u + pv, where u is the specific internal energy, p is the pressure, and v is specific volume, which is equal
Enthalpy
2026 video game
intentionally eliminated "wind-up motions" that resulted in smoother movements and inserted "more bold, memorable movements." During development, the programmers faced
Tomodachi Life: Living the Dream
Tomodachi_Life:_Living_the_Dream
Class of drugs
from the original on July 10, 2009. "Antithrombin (Recombinant) US Package Insert ATryn for Injection February 3, 2009" (PDF). Food and Drug Administration
Anticoagulant
Antimicrobial substance active against bacteria
phages, infect and kill bacteria primarily during lytic cycles. Phages insert their DNA into the bacterium, where it is transcribed and used to make new
Antibiotic
Temporary form of skin decoration
generally not subject to specific legislation and market controls, and may contain other undisclosed ingredients. The plants used to produce these pigments
Jagua_tattoo
Engine family by Ford Motor Company
procedure for out-of-warranty vehicles is to use the LOCK-N-STITCH aluminum insert and tool kit. 3-valve 5.4 L and 6.8 L engines built before October 9, 2007
Ford_Modular_engine
Patched software backdoor
that act together. A subsequent investigation found that the campaign to insert the backdoor into the XZ Utils project was a culmination of over two years
XZ_Utils_backdoor
Genetic alteration of a cell by uptake of genetic material from the environment
able to infect plants with A. tumefaciens and let the bacteria insert their chosen DNA into the genomes of the plants. Not all plant cells are susceptible
Genetic_transformation
Species of pitcher plant
ˌælboʊmɑːrdʒɪˈnɑːtə/ is a tropical pitcher plant native to Borneo, Peninsular Malaysia, and Sumatra. The specific epithet albomarginata, formed from the Latin
Nepenthes_albomarginata
Bacterium, genetic engineering tool
have been vastly explored in biotechnology as a means of inserting foreign genes into plants. Shortly after the Asilomar Conference, Marc Van Montagu
Agrobacterium_tumefaciens
Production of biochemicals which affect the growth of other organisms
and abundance within plant communities, and are also thought to be important in the success of many invasive plants. For specific examples, see black walnut
Allelopathy
Low molecular weight compound
plasmid (in Agrobacterium) or Ri plasmid (in Rhizobium), inserted by the bacterium into the plant genome. The opines are used by the bacterium as an important
Opine
Species of pitcher plant from Borneo and the Philippines
long spur is inserted at the base of the lid. N. campanulata is wholly glabrous. Plants spread by means of subterranean runners. Mature plants often form
Nepenthes_campanulata
Plant hormone
Native auxins Auxins (plural of auxin /ˈɔːksɪn/) are a class of plant hormones (or plant-growth regulators) with some morphogen-like characteristics. Auxins
Auxin
Data structure
last.left := p else last.mid := p p.splitchar := key[idx] idx := idx+1 // Insert remainder of key while idx < length(key) do p.mid := node() p.mid.splitchar
Ternary_search_tree
Group of natural pigments found in most organisms
lower smoking cessation rates in darker pigmented individuals. Melanocytes insert granules of melanin into specialized cellular vesicles called melanosomes
Melanin
2024 film by Coralie Fargeat
shoot" approach in its final month. The "lab shoot" was used to capture insert shots, typically assigned to a second unit with a reduced crew. The most
The_Substance
functionality is similar to that for back-compatibility with Xbox 360 games. Users insert the Xbox game disc into their Xbox One console to install the compatible
List of backward-compatible games for Xbox One and Series X/S
List_of_backward-compatible_games_for_Xbox_One_and_Series_X/S
Gene editing method
creating a targeted break at a specific location as designated by the crRNA and tracrRNA guide strands. Researchers can insert Cas9 and template RNA with
CRISPR_gene_editing
Species of Pentatomid insect
require a specific plant on which to feed. To obtain their food, stink bugs use their stylets to pierce the plant tissue to extract the plant fluids. In
Brown_marmorated_stink_bug
Form of message tampering
directly communicating with each other, where in actuality the attacker has inserted themselves between the two user parties. One example of a MITM attack is
Man-in-the-middle_attack
Computer-aided design and drafting software
discipline-specific enhancements such as: Advance Steel AutoCAD Architecture AutoCAD Electrical AutoCAD Map 3D AutoCAD Mechanical AutoCAD MEP AutoCAD Plant 3D
AutoCAD
American multinational food company
distributed in different editions over a period of 23 years. In 1945, Kellogg's inserted a prize in the form of pin-back buttons into each box of Pep cereal. Pep
Kellogg's
Family of DNA sequences found in prokaryotic organisms
homology-directed repair, or mismatch repair, to modify, insert, or delete genetic material at these specific cut sites. This technology has transformed fields
CRISPR
DNA sequencing method
into several consecutive short ones. The DNA of interest may be a plasmid insert, a PCR product or a fragment representing a gap when sequencing a genome
Primer_walking
Army dissolved The United States continued to train agents in the USA and insert them into Tibet until 1972 Dwight D. Eisenhower (January 20, 1953 – January
List of wars involving the United States in the 20th century
List_of_wars_involving_the_United_States_in_the_20th_century
Genus of ferns
cinnamon-colored fertile segments are inserted in the middle of the length, giving the plant its name. In their absence, the plant in all its stages appears similar
Claytosmunda
Plant disease
vector leafhopper feeds on the phloem of aster yellows-infected plants by inserting its straw-like mouthpart, a stylet, into the cell and extracting
Aster_yellows
Class of animals
digestion: The rocks are washed around in the stomach, helping to grind up plant matter. Fossil gastroliths have been found associated with both ornithopods
Reptile
2021 animated film by Shūkō Murase
two vocal tracks, "Möbius" for the opening animation and "TRACER" as an insert song. The music video for "Möbius" was released before the film's premiere
Mobile_Suit_Gundam:_Hathaway
Species of flowering plant
Trachelospermum asiaticum, the Asiatic jasmine, is a species of flowering plant in the family Apocynaceae and it is native to Asia. Its flowers resemble
Trachelospermum_asiaticum
Genetically engineered variety of rose
genes, and interfering with another. First, the researchers inserted a gene for the blue plant pigment delphinidin cloned from the pansy into a purplish-red
Blue_rose
Species of pitcher plant from Borneo
pitcher-plant, is a tropical pitcher plant endemic to Borneo, where it grows at elevations ranging from 0 to 500 m above sea level. The specific epithet
Nepenthes_northiana
Computer worm first discovered in 2010
domain-specific and it could be tailored as a platform for attacking modern SCADA and PLC systems (e.g., in factory assembly lines or power plants), most
Stuxnet
Substance to reduce friction, usually during sexual acts
ingredients. Some contain botanicals such as aloe vera or plant extracts. Warming lubricants contain specific ingredients to cause a sensation of warmth. Breathing
Personal_lubricant
Family of mostly succulent plants, adapted to dry environments
cactus (pl.: cacti, cactuses, or less commonly, cactus) is a member of the plant family Cactaceae (/kækˈteɪsi.iː, -ˌaɪ/), a family of the order Caryophyllales
Cactus
List of episodes from the 1970s television show, "Emergency!"
victim; he's in sinus rhythm." Early: "Administer two amps sodium bicarb and insert an airway. (Pause.) Start an IV Fifty-One, lactated Ringer's." McCall: "Squad
List_of_Emergency!_episodes
Species of pitcher plant from the Philippines
among known Nepenthes species. The plant itself does not grow very tall, however, and is not known to climb. The specific epithet robcantleyi honours Robert
Nepenthes_robcantleyi
Family of insects
one-tenth place Female Western Calligrapher on zinnia taking pollen and nectar. Insert shows entire visit at 3X speed. Some portions of main video shown at one-tenth
Hoverfly
Study of adaptation of an organism's physiology to environmental conditions
the membrane and in hot conditions, more saturated fatty acids are inserted. Plants can avoid overheating by minimising the amount of sunlight absorbed
Ecophysiology
Membrane enclosed compartments in chloroplasts and cyanobacteria
diacylglycerol and phosphatidylglycerol in photosynthesis: their specificity and evolution". J Plant Res. 117 (6): 495–505. Bibcode:2004JPlR..117..495S. doi:10
Thylakoid
the deltoid muscle of the upper arm. Statements in medication package inserts listing the frequency of side effects describe how often the effect occurs
List of common misconceptions about science, technology, and mathematics
List_of_common_misconceptions_about_science,_technology,_and_mathematics
PLANT SPECIFIC-INSERT
PLANT SPECIFIC-INSERT
Boy/Male
Gujarati, Hindu, Indian, Kannada, Malayalam, Marathi, Sanskrit
Little Plant; Small Plant
Surname or Lastname
English (chiefly Berkshire)
English (chiefly Berkshire) : from Middle English planke ‘plank’ (Late Latin planca). It is not clear how this word was applied as a surname: it may be a topographic name for someone who lived near a plank bridge over a stream, a metonymic occupational name for a carpenter, or a nickname for a thin person.North German : nickname for a cantankerous person, from Middle Low German plank ‘quarrel’, ‘discord’.North German : metonymic occupational name from Middle Low German plank ‘measure for liquids’.South German : topographic name from Middle High German plank ‘plank’, ‘palisade’.South German : nickname for a fair-haired person, from a variant of Middle High German blanc ‘light’, ‘shining’.
Boy/Male
Indian, Punjabi, Sikh
Plant
Boy/Male
English
Plant
Boy/Male
Hindu
One of krishnas incarnations. specific to education
Girl/Female
Hebrew
Plant.
Surname or Lastname
English
English : habitational name from Platt or Platt Bridge in Lancashire, named in Middle English with Old French plat ‘flat’, ‘thin’ (see Platte), in the dialect sense ‘plank bridge’.English : topographic name from Middle English plat ‘plot of land’, ‘piece of ground’ (Old English plætt).Jewish (Ashkenazic) : nickname from German platt ‘flat’.German : variant of Platte 3.
Boy/Male
Arthurian Legend
Father of Lancelot.
Girl/Female
Arabic
Precious; Lord of Specific Wood
Surname or Lastname
French (Planté)
French (Planté) : topographic name for someone living by an area of planted ground, a herb garden, shrubbery, or more specifically a vineyard.English : variant of Plant.
Girl/Female
Hebrew
Plant.
Boy/Male
French
From the flat land.
Boy/Male
French
From the flat land.
Surname or Lastname
English
English : perhaps a habitational name for someone who lived by a long strip of ground, Middle English langet (a derivative of lang ‘long’).
Girl/Female
Hebrew
Plant.
Girl/Female
Gujarati, Hindu, Indian, Kannada, Malayalam, Marathi, Oriya, Sindhi, Tamil, Telugu, Traditional
Sacred Plant; A Medicine Plant; Basil Plant
Male
Arthurian
, a king; father of Lancelot.
Girl/Female
Hebrew
Plant.
Surname or Lastname
English and French
English and French : metonymic occupational name for a gardener, in particular someone with a herb garden, from Middle English plant (Old English plante), Old French plante ‘herb’, ‘shrub’, ‘young tree’. In English it may also be a nickname for a tender or delicate individual, from the same word in a transferred sense.French : topographic name for a planted area, in particular one planted with herbs or vines. Compare Plantier.Jewish (eastern Ashkenazic) : unexplained.
Boy/Male
African French
Plant.
PLANT SPECIFIC-INSERT
PLANT SPECIFIC-INSERT
Boy/Male
Hindu, Indian, Marathi
Born of the Lotus
Boy/Male
Indian, Sanskrit
Fire Bannered
Girl/Female
Hindu
Goddess Lakshmi, Auspicious, Luster, Prosperity, Pratham, Shrestha
Boy/Male
Bengali, Gujarati, Hindu, Indian, Kannada, Malayalam, Marathi, Telugu
Lord of the Sacrificial Fire; Lord Fo the Sacrificial Fire
Boy/Male
Hindu
Partha, Arjun, Agni God, Fire
Girl/Female
Tamil
Wide load
Male
Chinese
swallow forest.
Girl/Female
Hindu, Indian, Marathi, Sanskrit
Pleasant; Delighted
Male
English
Variant spelling of English Darrell, DARREL means "from Airelle."
Boy/Male
French, German
Strong with a Sword
PLANT SPECIFIC-INSERT
PLANT SPECIFIC-INSERT
PLANT SPECIFIC-INSERT
PLANT SPECIFIC-INSERT
PLANT SPECIFIC-INSERT
v. t.
To mention or name, as a particular thing; to designate in words so as to distinguish from other things; as, to specify the uses of a plant; to specify articles purchased.
a.
Of or pertaining to a species; characterizing or constituting a species; possessing the peculiar property or properties of a thing which constitute its species, and distinguish it from other things; as, the specific form of an animal or a plant; the specific qualities of a drug; the specific distinction between virtue and vice.
a.
Eating, or subsisting on, plants; as, a plant-eating beetle.
n.
To introduce and establish the principles or seeds of; as, to plant Christianity among the heathen.
n.
A specific remedy. See Specific, a., 3.
n.
A slanting direction or plane; a slope; as, it lies on a slant.
a.
Alt. of Plano-
n.
To furnish with a fixed and organized population; to settle; to establish; as, to plant a colony.
a.
Combining forms signifying flat, level, plane; as planifolious, planimetry, plano-concave.
n.
To put in the ground and cover, as seed for growth; as, to plant maize.
n.
To set firmly; to fix; to set and direct, or point; as, to plant cannon against a fort; to plant a standard in any place; to plant one's feet on solid ground; to plant one's fist in another's face.
n.
A plan; an artifice; a swindle; a trick.
n.
To furnish, or fit out, with plants; as, to plant a garden, an orchard, or a forest.
imp. & p. p.
of Specify
a.
Specifying; definite, or making definite; limited; precise; discriminating; as, a specific statement.
v. t.
To interweave the strands or locks of; to braid; to plat; as, to plait hair; to plait rope.
a.
Exerting a peculiar influence over any part of the body; preventing or curing disease by a peculiar adaption, and not on general principles; as, quinine is a specific medicine in cases of malaria.
a.
Specific.