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Artificial plasmid
pBR322 is a plasmid and was one of the first widely used cloning vectors for E. coli. Created in 1977 in the laboratory of Herbert Boyer at the University
PBR322
technique used in recombinant DNA engineering where a plasmid (such as pBR322) is used to disable the expression of a gene. The inactivation of a gene
Insertional_mutagenesis
Small piece of maintainable DNA
15 kb in size. One of the earliest commonly used cloning vectors is the pBR322 plasmid. Other cloning vectors include the pUC series of plasmids, and a
Cloning_vector
Biological particle which transfers genetic material between cells
The pBR322 plasmid is one of the first plasmids widely used as a cloning vector.
Vector_(molecular_biology)
The tetA gene is also present in the widely used E. coli cloning vector pBR322, where it is often referred to by the name of its tetracycline-resistance
TetR
Set of methods in molecular biology
Diagram of a commonly used cloning plasmid; pBR322. It's a circular piece of DNA 4361 bases long. Two antibiotic resistance genes are present, conferring
Molecular_cloning
Cell line
non-cancerous adult male individual and subsequently transfected with a PBR322 based plasmid containing the coding region for SV40 in order to immortalise
MeT-5a
Number of copies of a plasmid in a cell
utilize mutated plasmids that replicate to high copy number. For example, pBR322 is a medium copy number plasmid (~20 copies/cell) from which several high
Plasmid_copy_number
Small DNA molecule within a cell
[clarification needed] In contrast, plasmids used in biotechnology, such as pUC18, pBR322 and derived vectors, hardly ever contain toxin-antitoxin addiction systems
Plasmid
Dense cluser of restriction sites in DNA
1977, scientists Francisco Bolivar and Raymond L. Rodriguez, built the pBR322 plasmid. While not being officially a multiple cloning site, this plasmid
Multiple_cloning_site
DNA or RNA sequence that matches its complement when read backwards
called insertional inactivation or insertional mutagenesis. For example, in PBR322 methylation at the tetracyclin resistant gene makes the plasmid liable to
Palindromic_sequence
link] genetics gene expression Transcription (genetics) translation λ phage pBR322 Lodes, M. J.; Dillon, D. C.; Houghton, R. L.; Skeiky, Y. A. (2004). Expression
Expression_cloning
DNA structure
recognize and cleave inverted repeat sequences within plasmids pVH51 and pBR322. The inverted repeat sequences in these plasmids displayed nicks on the
Cruciform_DNA
Genetically engineered chromosome derived from the DNA of yeast
Schematic of the pBR322 plasmid generated in the Boyer Lab at UC San Francisco by Bolivar and Rodriguez in 1972. It is one of the first and most widely
Yeast_artificial_chromosome
American professor of biology
molecule, pBR322, the first general purpose molecular cloning vector approved by the National Institute of Health Guidelines. The abbreviation, “pBR322,” refers
Raymond_L._Rodriguez
Secondary cell line
pulmonary endothelium of a human male and subsequently transfected with a PBR322 based plasmid containing the coding region for SV40 in order to immortalise
HuLEC-5a
Enzyme
5′-GGCC-3′) was made into Escherichia coli (E.coli) in the plasmid vector pBR322. The gene was extracted from a single EcoRI fragment and a single HindIII
HaeIII
Enzyme
were found in some phages (bacteriophage Mu group) and plasmids (pSC101, pBR322). Recently, high throughput mapping of DNA gyrase sites in the Escherichia
DNA_gyrase
Ability of a cell to take up DNA
for determination of transformation efficiency in Escherichia coli are pBR322 or other similarly sized or smaller vectors, such as the pUC series of vectors
Transformation_efficiency
acyltransferase - Parkinson's disease - Partial cleavage stimulation factor domain - pBR322 - PCR - pedigree - peptide - peptide-transporting ATPase - peptide bond
Index of molecular biology articles
Index_of_molecular_biology_articles
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