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Mechanism of nuclear organization
Loop extrusion is a major mechanism of nuclear organization. It is a dynamic process in which structural maintenance of chromosomes (SMC) protein complexes
Loop_extrusion
Protein complex
condensins, loop extrusion has recently attracted the most attention. The concept of loop extrusion, where condensins actively "extrude" DNA to form loops, was
Condensin
Protein complex that regulates the separation of sister chromatids during cell division
process of loop extrusion, cohesin moves actively along the two DNA double helices, translocating one of them with respect to the other. Thus, the loop can become
Cohesin
Spatial distribution of chromatin within a cell nucleus
breathing. At intermediate scales, an active process of loop extrusion creates dynamic loops and Topologically Associating Domains (TADs). Each human
Nuclear_organization
Self-interacting genomic region
simulations have shown that chromatin loop extrusion driven by cohesin motors can generate TADs. In the loop extrusion model, cohesin binds chromatin, pulls
Topologically associating domain
Topologically_associating_domain
Complex of DNA and protein in eukaryotic cells
with loops forming and disappearing. The loops are regulated by two main elements: Cohesins, protein complexes that generate loops by extrusion of the
Chromatin
Transcription factor
loop extrusion. Loop extrusion is a mechanism by which SMC complexes such as cohesin translocate along chromatin and progressively enlarge DNA loops.
CTCF
Process in mitosis and meiosis
energy from ATP hydrolysis to form DNA loops. Among the various models proposed for loop formation, the loop extrusion mechanism attracts much attention.
Chromosome_condensation
complex, and Wadjet are capable of extruding DNA loops in an ATP-dependent manner. During loop extrusion, the ATPase cycle of the SMC subunits is thought
SMC_protein
Medical condition
de Lange syndrome mutations in NIPBL can impair cohesin-mediated DNA loop extrusion". PNAS. 119 (18) e2201029119. Bibcode:2022PNAS..11901029P. doi:10.1073/pnas
Cornelia_de_Lange_syndrome
3D printing process that melts thermoplastic material from a spool of filament
feedback loop. This is similar to ambient heating of a room. FFF and the other technologies of additive manufacturing by material extrusion (EAM) techniques
Fused_filament_fabrication
Region within a prokaryotic cell containing genetic material
SMC complexes from different organisms differ in the mechanism of loop extrusion. Single molecule fluorescence microscopy of MukBEF in E. coli suggests
Nucleoid
Physicist specializing in biophysics and soft matter
contribution of his group is the conserved-current loop extrusion (CCLE) model, which interprets loop-extruding cohesin as a conserved current to self-consistently
Simon_G._J._Mochrie
British biochemist
Kim Nasmyth explaining loop extrusion with a climbing rope
Kim_Nasmyth
Institute of Delft University of Technology
Ganji et al for their publication entitled 'Real-time imaging of DNA loop extrusion by condensin' appeared in Science in 2018. "Home - Kavli Institute of
Kavli Institute of Nanoscience
Kavli_Institute_of_Nanoscience
Industrial container
film. This film is then stretched orientedly (a process known as tape extrusion) to form high-tensile tape yarn. This orienting process aligns the polymer
Flexible intermediate bulk container
Flexible_intermediate_bulk_container
Protein-coding gene in humans
STAG2 has been shown to interact with STAG1. Cohesion folds by DNA loop extrusion and this cohesion consists of SMC1, SMC3, RAD21, and either STAG1 or
STAG2
transcription factors. Three-dimensional organisation with chromatin loop extrusion provides the basis for topologically associated domains (TADs). TADs
Range Extender (REX) DNA element
Range_Extender_(REX)_DNA_element
Genomic analysis technique
property of underlying biological mechanisms, which defines TADs as loop extrusions, compartmentalizations, or any dynamic genomic pattern rather than
Hi-C (genomic analysis technique)
Hi-C_(genomic_analysis_technique)
In mammalian biology, insulated neighborhoods are chromosomal loop structures formed by the physical interaction of two DNA loci bound by the transcription
Insulated_neighborhood
Deformation mechanism in crystallines
dislocation loops at the surface cause the irreversible/persistent surface step associated with slip-bands. Surface relief through extrusion occurs on the
Slip_bands_in_metals
Laboratory tool for bacteria
of a triangle with a handle. Another variant is a rod bent in L-shape. Extrusion molded versions can be T-shaped. Drop cells or bacteria at the center
Cell_spreader
Part of the thermoplastic elastomer family of polymers
standard thermoplastic processing methods, such as injection molding and extrusion. Coprocessing with other polymers can include coextrusion, molding, and
Thermoplastic_vulcanizates
Mechanical, toroid gasket that seals an interface
cross-sectional diameter, and radial clearance. O-rings can be produced by extrusion, injection molding, pressure molding, or transfer molding. The first patent
O-ring
Methods used for additive 3D manufacturing
three-dimensional object via additive manufacturing. Techniques include jetting, extrusion, additive friction stir deposition, powder bed fusion, binder jetting
3D_printing_processes
Swiss researcher
critical regulator of IgH locus contraction that facilitates chromatin loop extrusion across the entire locus. He is a member of the editorial board for Immunity
Meinrad_Busslinger
DNA structure
range of cellular proteins were characterized. The mechanism of cruciform extrusion occurs through the opening of double stranded DNA to allow for intrastrand
Cruciform_DNA
Soft water-rich polymer gel
Goodyear Baekeland Hayward Braconnot Applications Industrial production Extrusion Blow molding Applied coatings Protective Coatings 3D printing Consumer
Hydrogel
Short circular highly elastic rubber loop
A rubber band (also known as an elastic, gum band or lacky band) is a loop of rubber, usually ring or oval shaped, and commonly used to hold multiple objects
Rubber_band
Species of flowering plant with edible seeds
improved through treatments such as germination, dehulling, and heat. Extrusion is a very good heat technique to destroy secondary legume components since
Chickpea
Thermoplastic polymer
Melting process of polypropylene can be achieved via extrusion and molding. Common extrusion methods include production of melt-blown and spun-bond
Polypropylene
Type of retail packaging
microbial growth. Three main processes are used: Coextrusion – simultaneous extrusion of polymers, often with tie layers Lamination – bonding prefabricated
Multilayered_packaging
Recycling of bottles made of polyethylene terephthalate
integrate a screen changer into the extrusion line. This can be in a pelletizing, sheet extrusion or strapping tape extrusion line. The success of any recycling
PET_bottle_recycling
Heat-transfer device that employs phase transition
aluminum/ammonia VCHP for spacecraft thermal control. The heat pipe is an aluminum extrusion, similar to that shown in the first figure. The bottom flanged area is
Heat_pipe
Bottle used for selling craft beer and other drinks
Die cutting Die forming (plastics) Electronic article surveillance Extrusion Extrusion coating Flame treatment Glass production Graphic design Hazard analysis
Growler_(jug)
Method of representing a 3D object by defining the limits of its volume
richer operation set. In addition to the Boolean operations, B-rep has extrusion (or sweeping), chamfer, blending, drafting, shelling, tweaking and other
Boundary_representation
Die cutting Die forming (plastics) Electronic article surveillance Extrusion Extrusion coating Flame treatment Glass production Graphic design Hazard analysis
List of bottle types, brands and companies
List_of_bottle_types,_brands_and_companies
Wine packaged in a bag-in-box arrangement
Die cutting Die forming (plastics) Electronic article surveillance Extrusion Extrusion coating Flame treatment Glass production Graphic design Hazard analysis
Box_wine
Device used to protect the wearer's face from hazards
methods are used to manufacture face shields: extrusion and injection molding. Face shields cut from extrusion sheets provide better impact resistance than
Face_shield
Centralized heat distribution system
egyr.2021.08.161. ISSN 2352-4847. S2CID 240180109. Doyle, Lucía (2021). "Extrusion foaming behavior of polybutene-1. Toward single-material multifunctional
District_heating
Specialized materials engineered from natural or synthetic fibers
from proteins or bacterial cellulose are commonly drawn out during an extrusion and spun into a stronger thread. By cultivating plant and bacterial species
Biotextile
Pore-forming membrane protein
allow protons to leave cells. Their function thus appears to be acid extrusion from cells. Another important function occurs in phagocytes (e.g. eosinophils
Ion_channel
Common synthetic polymer
cables. PVC is chosen because of its good electrical insulation, ease of extrusion, and resistance to burn. In a fire, PVC can form hydrogen chloride fumes;
Polyvinyl_chloride
Kitchen cooking appliance
visible as a spark. The plasma and the tines may then form a conductive loop, which may be a more effective antenna, resulting in a longer lived spark
Microwave_oven
Nucleic acid sequence
cruciform extrusion. DNA in the region of the inverted repeat unwinds and then recombines, forming a four-way junction with two stem-loop structures
Inverted_repeat
1986 breakup of American orbiter
O-rings to be extruded from their seats rather than compressed. This extrusion was judged to be acceptable by NASA and Morton Thiokol despite concerns
Space Shuttle Challenger disaster
Space_Shuttle_Challenger_disaster
Aqueous solution of hydrogen chloride
oxide scale from iron or steel before subsequent processing, such as extrusion, rolling, galvanizing, and other techniques. Technical quality HCl at
Hydrochloric_acid
Tool used on products on conveyor belts
engineered. With construction ranging from 1⁄8 in (3.2 mm) thick aluminum extrusion to 11 gauge fabricated stainless steel, air knives can weigh 1 lb/ft to
Air_knife
Class of polymers
at a relatively low molecular weight so they can be melt processed by extrusion or injection-molding. The molded articles are then thermally treated for
Polyamide-imide
Water-soluble domain of transmembrane ABC transporters
systems) for function. In contrast, export systems are involved in the extrusion of noxious substances, the export of extracellular toxins and the targeting
ATP-binding domain of ABC transporters
ATP-binding_domain_of_ABC_transporters
Fishing line made from a single fiber of plastic
strands of line, which is then spun into spools of various thicknesses. The extrusion process controls not only the thickness of the line but its test as well
Monofilament_fishing_line
Additive process used to make a 3D object
models—is fused deposition modeling, a special application of plastic extrusion, developed in 1988 by S. Scott Crump and commercialized by his company
3D_printing
Moving staircase
In a factory, handrails are constructed by feeding rubber through an extrusion machine to produce layers of the required size and type in order to match
Escalator
Mechanism that controls the pressure of a breathing gas supply for diving
tools. Conversely, failing to tighten sufficiently can lead to O-ring extrusion under pressure and a major loss of breathing gas. This can be a serious
Diving_regulator
Antiporter membrane protein that removes calcium from cells
Costa E, Wroblewski JT (Feb 1994). "Glutamate impairs neuronal calcium extrusion while reducing sodium gradient". Neuron. 12 (2): 295–300. doi:10
Sodium-calcium_exchanger
Systems for conveying fluids
typically formed via casting or welding, whereas a tube is made through extrusion. Pipe normally has thicker walls and may be threaded or welded, while
Plumbing
Very long-chain polyethylene with high impact strength
monomers. UHMWPE is processed variously by compression moulding, ram extrusion, gel spinning, and sintering. Several European companies began compression
Ultra-high-molecular-weight polyethylene
Ultra-high-molecular-weight_polyethylene
Company in Paris, France
cover steel casting (static and centrifugal casting), forging (close-die, extrusion, welding), and provide ‘ready-to-assemble’ gear grinding and cutting,
Manoir_Industries
Industrially formulated edible substance
and flavourings. UPFs typically undergo processes such as moulding, extrusion, hydrogenation, or frying. Ultra-processed foods first became ubiquitous
Ultra-processed_food
Process which produces red blood cells
approximately 120 days. During this maturation process, there is nuclear extrusion – i.e. mature erythrocytes have no nucleus. Nucleated red blood cells
Erythropoiesis
observed slight maxillary first molars intruded and slight first premolars extrusion. This study also saw increase of lower anterior facial height by 2.2 mm
Pendulum_appliance
Type of program in computer graphics
include point sprite generation, geometry tessellation, shadow volume extrusion, and single pass rendering to a cube map. A typical real-world example
Shader
Drinking cup intended for a single use
Die cutting Die forming (plastics) Electronic article surveillance Extrusion Extrusion coating Flame treatment Glass production Graphic design Hazard analysis
Disposable_cup
Processed food made from grain
branded and ready-to-eat cold cereals (many produced via the process of extrusion) appeared around the late 19th century. These processed, precooked, packaged
Breakfast_cereal
Essential nutrient
ATP2B1 (PMCA1b), a plasma membrane calcium ATPase involved in calcium extrusion from the cell; and the S100A family of genes, which encode calcium-binding
Vitamin_D
Method of reusing or reprocessing used clothing, fibrous material and rags
The dried plastic remnants are molded into PET pellets and then undergo extrusion to create new fibers. These new fibers can then be used to create new
Textile_recycling
Protein family
at lower concentrations of arsenite and enhancing the rate of arsenite extrusion. Carlin A, Shi W, Dey S, Rosen BP (February 1995). "The ars operon of
Ars_operon
Group of transport proteins
by transmembrane regions four through seven. Additionally, bumetanide, a loop diuretic, was found to bind to transmembrane regions 2 through 7, 11, and
Na–K–Cl_cotransporter
Cell division producing haploid gametes
animals, three of the four meiotic products are typically eliminated by extrusion into polar bodies, and only one cell develops to produce an ovum. Because
Meiosis
Israeli cleantech company
used with conventional manufacturing equipment for injection molding, extrusion, compression molding, 3D printing, and rotomolding to produce products
UBQ_Materials
support the counter. horse 1. A metal bar (sometimes a shaped aluminium extrusion), running athwartships, to which a sheet is attached with a traveller
Glossary of nautical terms (A–L)
Glossary_of_nautical_terms_(A–L)
Early synthetic polymer developed as a textile fiber
components previously cast in metal. Engineering-grade nylon is processed by extrusion, casting, and injection molding. Type 6,6 Nylon 101 is the most common
Nylon
Process of keeping all parts of a spacecraft within acceptable temperature ranges
constant-conductance heat pipes (CCHPs) or axial-groove heat pipes, are aluminum extrusions with ammonia used as the working fluid. Typical applications include payload
Spacecraft_thermal_control
function and may form an intramembranous pore/loop-like structure. These carriers function primarily in Ca2+ extrusion. The phylogenetic tree for the CaCA family
Calcium:cation_antiporter
Chemical reactions and pathways
cobalt-precorrin-5 + S-adenosyl-L-homocysteine The scene is now set for the extrusion of the two-carbon fragment corresponding to the acetate released in the
Cobalamin_biosynthesis
switching the direction of mobile phase flow through the column. The extrusion of stationary phase from the column at the end of a separation experiment
Countercurrent_chromatography
Device that mechanically joins objects
frequent adjustments. T-slot fasteners: Designed for T-slotted aluminium extrusions, T-slot fasteners provide a secure and adjustable connection for framing
Fastener
Cellulose-based semi-synthetic fiber
process. The filaments are allowed to pass through a coagulation bath after extrusion from the spinneret holes. Drawing. The rayon filaments are stretched,
Rayon
cases the properly prepared hole in sheetmetal uses an extruded hole. The extrusion forms a lead-in and extra thread length for improved retention. Thread
List_of_screw_and_bolt_types
Light-conducting fiber
by drawing, while plastic fibers can be made either by drawing or by extrusion. Optical fibers typically include a core surrounded by a transparent cladding
Optical_fiber
Class of chemical substance
of the mechanical properties since most processing techniques (e.g., extrusion and pelletization) expose the MOFs to substantial mechanical compressive
Metal–organic_framework
Industry involved with discovery, development, production and marketing of drugs
were developed and found wide acceptance in combination therapy, including loop diuretics (Lasix/furosemide, Hoechst Pharmaceuticals, 1963), beta blockers
Pharmaceutical_industry
Cylindrical container for storing pressurised gas
requirements of the end user. Section of die with billet inserted Cold extrusion process Extrusion product before trimming Section after closure of the top end
Gas_cylinder
Type of plastic
thermoplastic to a thermoset. Cross-linking is accomplished during or after the extrusion of the tubing. The required degree of cross-linking, according to ASTM
Cross-linked_polyethylene
Protein
Fullmer CS, et al. (March 1992). "Intestinal calcium transport and calcium extrusion processes at the basolateral membrane". The Journal of Nutrition. 122
Calbindin
American engineer, inventor, designer, entrepreneur (born 1950)
along with patents for other 3D printing technologies (piezo transducer, extrusion, lithography, surface tension, and pin array) and computer assisted manufacturing
William_Masters_(engineer)
Linear crystallographic defect or irregularity
plasticity is transmitted by screw dislocations. Where PSB's meet the surface, extrusions and intrusions form, which under repeated cyclic loading, can lead to
Dislocation
General principles ISO 527-2:2012 Part 2: Test conditions for moulding and extrusion plastics ISO 527-3:2018 Part 3: Test conditions for films and sheets ISO
List_of_ISO_standards_1–1999
Long continuous length of interlocked fibres
to length measures. Cut-length only applies to staple fiber. Filament extrusion is sometimes referred to as "spinning," but most people equate spinning
Yarn
Flat structure to transport goods
molding Thermoforming Compression molding Rotational molding Profile extrusion Paper pallets, also referred to as "ecopallets", are often used for light
Pallet
Concept of collecting solar power in outer space and distributing it to Earth
such as SpiderFab might allow mass launch of raw materials for local extrusion. Method of installation / Transportation of Material to Energy Collection
Space-based_solar_power
Category of polymers, in which the monomers are joined together by ester links
temperatures and involves the removal of water or methanol byproducts. Extrusion: Once the polymerization is complete, the molten polyester is extruded
Polyester
to protect sensitive components from puncture damage, and an aluminum extrusion to absorb impact energy. The new shields, which decrease vehicle range
List of thermal incidents in electric vehicles
List_of_thermal_incidents_in_electric_vehicles
Group of British electric multiple unit trains
common underframe, created by seam-welding a number of aluminium alloy extrusions, is covered by body panels and topped by a single piece roof, again made
Bombardier_Electrostar
Hybrid electric car by Honda
lightweight aluminum monocoque, reinforced in key areas with aluminum extrusions joined at cast aluminum lugs. Stamped aluminum panels are welded onto
Honda_Insight
Geographic information system maintained by Esri
the 3D Analyst License. In the layer properties of ArcScene there is an Extrusion function which allows the user to exaggerate features three dimension-ally
ArcGIS
German-American neurologist and corporate executive
Enhancement of Potassium-Chloride Cotransporter KCC2–Mediated Chloride Extrusion in Peripheral Neuropathic Pain". JAMA Neurology. 71 (5): 640–645. doi:10
Wolfgang_Bernhard_Liedtke
Ultra-luxury flagship automobile
work, by hand, in keeping with the Rolls-Royce tradition. The aluminium extrusions that are used to construct the aluminium spaceframe are produced in Norway
Rolls-Royce_Phantom_VII
Landform near Mammoth Mountain, California, USA
from the Long Valley magma chamber were confined within the caldera with extrusions of relatively hot (crystal-free) rhyolite 700,000 to 600,000 years ago
Long_Valley_Caldera
Lightweight black carbon residue
pressure of the extruding process hold the charcoal together. If the extrusion is made from raw wood material, the extruded logs are subsequently carbonized
Charcoal
LOOP EXTRUSION
LOOP EXTRUSION
Girl/Female
Arabic, Muslim
Look
Girl/Female
Gujarati, Hindu, Indian
Look
Surname or Lastname
English
English : metonymic occupational name for a cooper, from Middle English coupe ‘tub’, ‘container’ (see Cooper). In some cases the surname may have been derived from a pub or house sign.Dutch : from koop ‘purchase’, ‘bargain’, hence a nickname for a haggler or a metonymic occupational name for a merchant.
Surname or Lastname
English (Somerset)
English (Somerset) : habitational name from Look in Puncknowle, Dorset, named in Old English with lūce ‘enclosure’.English : possibly a variant of Luck 3.Northern English and Scottish : from a vernacular pet form of Lucas.Dutch (van Look) : topographic name from look ‘enclosure’ or habitational name from a place named with this word.Thomas Look (b. c. 1622) was in Lynn, MA, by 1646. His son, also called Thomas (b. 1646), moved to Martha’s Vineyard about 1670.
Boy/Male
Hindu
Flower
Boy/Male
Hindu, Indian, Rajasthani, Sindhi, Traditional
Look; Beauty; Appearance
Boy/Male
Dutch, German, Hebrew
God will Multiply; God will Add
Boy/Male
Hindu, Indian
Look
Male
Dutch
, Jehovah's gift (or grace).
Boy/Male
Hebrew
God will multiply.
Girl/Female
Hindu
Look, Blessed with beauty, Shape, Beauty
Boy/Male
Arabic
The Biblical Lot is the English Language Equivalent
Male
French
French form of Latin Lupus, LOUP means "wolf."
Surname or Lastname
English
English : possibly from the Old Norse personal name Tópi, Túpi, a short form of a personal name formed with þórr, name of the Norse god of thunder (see Thor) + a second element with initial b-, for example björn ‘bear’, ‘warrior’. On the other hand, the name is found mainly in Dorset and Devon, which are far from areas of Scandinavian settlement.
Boy/Male
British, English
Barrel Maker
Boy/Male
Indian, Sanskrit
Natural; Original; Innate; Simply; Loop
Surname or Lastname
Dutch
Dutch : from a short form of the Germanic personal name Robrecht.Altered spelling of German Rupp.English : variant spelling of Roope.
Girl/Female
Tamil
Look, Blessed with beauty, Shape, Beauty
Surname or Lastname
North German
North German : habitational name from any of several places called Loose or Loosey.North German : from a short form of Nikolaus, German form of Nicholas.Dutch : nickname from the adjective loos ‘cunning’, ‘artful’, ‘guileful’.English : variant spelling of Loose.
Boy/Male
Bengali, Indian
Loop; Autumn
LOOP EXTRUSION
LOOP EXTRUSION
Girl/Female
Latin
Fruitful orchard, as Mount Carmel in Palestine.
Surname or Lastname
English (Yorkshire and Lancashire)
English (Yorkshire and Lancashire) : habitational name from any of various minor places so called, from Old English (e)ald ‘old’ + feld ‘open country’.
Girl/Female
Latin
Grace.
Female
Greek
(Δάμαλις) Greek name DAMALIS means "calf."
Boy/Male
Welsh
White fortress.
Girl/Female
Irish American
Modern phonetic form of the Irish name Caitlin from Catherine meaning pure.
Female
Irish
From the Italian city name, Loreto, LORETO means "laurel wood." The city has been a Catholic place of pilgrimage since the 14th century, for it is where the Shrine of the Holy House is. According to legend, after the fall of Jerusalem, a basilica was erected over the Virgin Mary's house. After a threat of destruction by the Turks, angels carried the house from Nazareth to Tersatto, Croatia, then across the Adriatic to a forest near Recantai, and finally to Loreto. In use by the English and Irish.
Girl/Female
Tamil
Srinandini | ஸà¯à®°à¯€à®¨à®¾à®¨à¯à®¤à¯€à®¨à¯€
Boy/Male
Finnish, German
Mighty Warrior
Girl/Female
Biblical
Doing; my doing.
LOOP EXTRUSION
LOOP EXTRUSION
LOOP EXTRUSION
LOOP EXTRUSION
LOOP EXTRUSION
n.
A curve of any kind in the form of a loop.
n.
The act of looking; a glance; a sight; a view; -- often in certain phrases; as, to have, get, take, throw, or cast, a look.
n.
See Loon, the bird.
v. t.
To look at; to turn the eyes toward.
v. t.
To break over the poop or stern, as a wave.
n.
Any one of several aquatic, wed-footed, northern birds of the genus Urinator (formerly Colymbus), noted for their expertness in diving and swimming under water. The common loon, or great northern diver (Urinator imber, or Colymbus torquatus), and the red-throated loon or diver (U. septentrionalis), are the best known species. See Diver.
n.
A ring; a circular band; anything resembling a hoop, as the cylinder (cheese hoop) in which the curd is pressed in making cheese.
v. t.
To bind or fasten with hoops; as, to hoop a barrel or puncheon.
n.
Expression of the eyes and face; manner; as, a proud or defiant look.
v. t.
To confine in a coop; hence, to shut up or confine in a narrow compass; to cramp; -- usually followed by up, sometimes by in.
v. t.
To influence, overawe, or subdue by looks or presence as, to look down opposition.
v. t.
To beat in the game of loo by winning every trick.
v. i.
To seem; to appear; to have a particular appearance; as, the patient looks better; the clouds look rainy.
n.
See 1st Loop.
v. i.
To direct the attention (to something); to consider; to examine; as, to look at an action.
v. t.
To let hang down; as, to lop the head.
pl.
of Trou-de-loup
n.
Hence; Appearance; aspect; as, the house has a gloomy look; the affair has a bad look.
v. t.
To make a loop of or in; to fasten with a loop or loops; -- often with up; as, to loop a string; to loop up a curtain.
v. t.
To express or manifest by a look.