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G418

G418

Chemical compound

G418 (geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by Micromonospora rhodorangea. G418 blocks polypeptide

G418

Gentamicin

Antibiotic medication

position 6' is methylated to form the pharmacologically active intermediate G418 G418 then undergoes dehydrogenation and amination at the C6' position by the

Gentamicin

Transfection

Process of introducing nucleic acids into eukaryotic cells

agents for selecting stable transfection Agent Selectable marker Geneticin (G418) neomycin resistance gene NeoR Puromycin Puromycin N-acetyltransferase (PURO)

Transfection

HEK 293 cells

Cell line derived from human embryonic kidney cells

transfection used to create 293T (involving plasmid pRSV-1609) conferred neomycin/G418 resistance and expression of the tsA1609 allele of SV40 large T antigen;

HEK 293 cells

HEK_293_cells

Neomycin

Type of antibiotic

Biomembranes. 979 (1): 105–12. doi:10.1016/0005-2736(89)90529-4. PMID 2537103. "G418/neomycin-cross resistance?". Archived from the original on 25 June 2009.

Neomycin

Shinya Yamanaka

Japanese stem cell researcher (born 1962)

pluripotent stem cells which induce b-geo expression, which in turn gives rise to G418 resistance; this resistance helps us identify the iPS cells in culture. Moreover

Shinya Yamanaka

Shinya_Yamanaka

Kanamycin A

Antibiotic

Mammalian cells, yeast, and other eukaryotes acquire resistance to geneticin (= G418, an aminoglycoside antibiotic similar to kanamycin) when transformed with

Kanamycin A

Kanamycin_A

Musica notturna delle strade di Madrid

String quintet by Luigi Boccherini

are arrangements of the Ritirata (Retreat) movement incorporated to the (G418) piano quintet, and the (G453) guitar quintet. The Night Music of the Streets

Musica notturna delle strade di Madrid

Musica_notturna_delle_strade_di_Madrid

Pliosaurus

Extinct genus of marine reptiles

discovered in Stretham, while the second is a single tooth catalogued as LEICT G418.1965.108, discovered in Ely, which are stored at the Sedgwick Museum of Earth

Pliosaurus

Protein synthesis inhibitor

Inhibitors of translation

well as causing incomplete chains to be released. Geneticin, also called G418, inhibits the elongation step in both prokaryotic and eukaryotic ribosomes

Protein synthesis inhibitor

Protein_synthesis_inhibitor

Reporter gene

Technique in molecular biology

TLC. Selectable markers (e.g., Neo) confer antibiotic resistance (e.g., to G418), ensuring only transformed cells survive in selective media. In the case

Reporter gene

Reporter_gene

Protein-coding gene in the species Homo sapiens

the Lipofectin, and the cells are maintained in the presence of 0.5 mg/ml G418. The expressions seen in COS-7 cells from the G6b isoforms come from the

G6B

BOSC23

Human cell line

an important safety feature.[citation needed] BOSC 23 carries neomycin/G418 resistance derived from its parental line 293T, and also hygromycin and mycophenolic

BOSC23

Sonoporation

Technique in molecular biology

using sonoporation. This successful plasmid DNA transfection conferring G418 antibiotic resistance ultimately led to further in vitro studies that hinted

Sonoporation

Julian Davies (microbiologist)

British microbiologist (1932–2025)

enzymes PstI and KpnI and the npt gene conferring resistance to the antibiotic G418 widely used in research. Davies was one of the early voices warning that

Julian Davies (microbiologist)

Julian_Davies_(microbiologist)

Site-specific recombinase technology

Genome engineering tools

selectable marker, typically by insertion of a hygtk ([+/-]) cassette providing G418 resistance. In the following "knock-in" step, the tagged genomic sequence

Site-specific recombinase technology

Site-specific_recombinase_technology

RNLB Cecil Paine

A. C. Paine Stations Wells-next-the-Sea Cost £7,462 0s 3d Yard number G&G418 Acquired 25 July 1945 Decommissioned 1965 In service 1945 to 1965 for RNLI

RNLB Cecil Paine

RNLB_Cecil_Paine

Spizellomyces punctatus

Chytrid fungal species

have been tested. The growth of S. punctatus is not inhibited by Geneticin (G418), Puromycin, and Phleomycin D10 (Zeocin) up to 800 mg/L. 200 mg/L Hygromycin

Spizellomyces punctatus